This is especially accurate for immunohistochemistry by which 10% NBF is shown to be a poor fixative (Fig. two). Some issues with using ten% NBF for a fixative are already corrected by the introduction of other experimental approaches such as antigen retrieval (Shi et al. 2001) and the usage of little amplicons in RT-Q-PCR (Steg et al. 2006, 2007).
d) If using a processor that may accommodate a number of baskets vertically in a single retort, make sure that all baskets are adequately seated.A single intriguing anecdote, the entire process of clearing was initially termed as such, as the reagents utilized for this phase Possess a large index of refraction and may render the tissue clear.We uti
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